Quantitative characterization of protein–protein complexes involved in base excision DNA repair
نویسندگان
چکیده
منابع مشابه
Quantitative characterization of protein–protein complexes involved in base excision DNA repair
Base Excision Repair (BER) efficiently corrects the most common types of DNA damage in mammalian cells. Step-by-step coordination of BER is facilitated by multiple interactions between enzymes and accessory proteins involved. Here we characterize quantitatively a number of complexes formed by DNA polymerase β (Polβ), apurinic/apyrimidinic endonuclease 1 (APE1), poly(ADP-ribose) polymerase 1 (PA...
متن کاملBase excision DNA repair and cancer
Transformed cells can develop drug resistance via repair mechanisms that counteract the DNA damage from chemotherapy or radiation therapy. Disruption of DNA repair pathways can cause mis-repair that is cytotoxic [1]. Specific DNA repair inhibitors might thus be combined with DNA-damaging agents for improved therapy. In addition, some cancer cells have a reduced repertoire of DNA damage response...
متن کاملDNA glycosylases in the base excision repair of DNA.
A wide range of cytotoxic and mutagenic DNA bases are removed by different DNA glycosylases, which initiate the base excision repair pathway. DNA glycosylases cleave the N-glycosylic bond between the target base and deoxyribose, thus releasing a free base and leaving an apurinic/apyrimidinic (AP) site. In addition, several DNA glycosylases are bifunctional, since they also display a lyase activ...
متن کاملBiochemical characterization and DNA repair pathway interactions of Mag1-mediated base excision repair in Schizosaccharomyces pombe
The Schizosaccharomyces pombe mag1 gene encodes a DNA repair enzyme with sequence similarity to the AlkA family of DNA glycosylases, which are essential for the removal of cytotoxic alkylation products, the premutagenic deamination product hypoxanthine and certain cyclic ethenoadducts such as ethenoadenine. In this paper, we have purified the Mag1 protein and characterized its substrate specifi...
متن کاملInvolvement of flap endonuclease 1 in base excision DNA repair.
Base excision repair can proceed in either one of two alternative pathways: a DNA polymerase beta-dependent pathway and a proliferating cell nuclear antigen (PCNA)-dependent pathway. Excision of an apurinic/apyrimidinic (AP) site by cutting the phosphate backbone on its 3' side following incision at its 5' side by AP endonuclease is a prerequisite to completion of these repair pathways. Using a...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2015
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkv569